Start from the historic Barrio Santa Cruz and head through the Jardines de Murillo towards the Antigua Fábrica de Tabacos and Seville University. Your guide will help you get oriented in the city and show the vast majority of Seville’s landmarks.
Continue to Parque María Luisa, centerpiece of the 1929 Iberian-American Exposition. From there, head down to the river and check out a part of Triana and the Torre del Oro bullring.
Next, head north to the Cartuja, site of the 1992 Exposition, where you'll see the Monasterio de la Cartuja, before heading back to the center and Alameda.
Explore the nightlife hotspot and wind your way through the streets and alleys of Seville center, stopping at the legendary Campana Café to get your bearings for the infamous “setas” (mushrooms). End with a quick pedal around Santa Cruz, biking past the cathedral and Alcazares.